Full-length sequence of the cDNA for human erythroid beta-spectrin.

Complete nucleotide sequence of the murine erythroid beta-spectrin cDNA and tissue-specific expression in normal and jaundiced mice.

Spectrin, a heterodimer of alpha and beta subunits, is an essential component of the red blood cell membrane skeleton. The jaundiced (ja/ja) mutation causes a severe hemolytic anemia in mice and is mapped to the erythroid beta-spectrin locus (Spnb-1) on chromosome 12. As a prerequisite for determining the molecular defect of the jaundiced mutation, we have cloned and sequenced the complete muri...

Nucleotide sequence of the full length cDNA encoding for human type II procollagen.

Type II collagen is a homotrimeric molecule composed of three identical al(II) collagen chains that are synthesized as precursor procollagens with aminoand carboxy-terminal propeptides that are removed extracellularly. Mutati'ons in the ol(II) .chain result in disproportionate dwarfing disorders in the spectrum of skeletal dysplasias,' Hitherto, only the propeptides' sequences have been publish...

An Integrated Database System for Full-Length Human cDNA in NEDO Full-Length Human cDNA Sequencing Project

Golgi spectrin: identification of an erythroid beta-spectrin homolog associated with the Golgi complex

Spectrin is a major component of a membrane-associated cytoskeleton involved in the maintenance of membrane structural integrity and the generation of functionally distinct membrane protein domains. Here, we show that a homolog of erythrocyte beta-spectrin (beta I sigma*) co-localizes with markers of the Golgi complex in a variety of cell types, and that microinjected beta-spectrin codistribute...

Cloning and nucleotide sequence of a mouse erythrocyte beta-spectrin cDNA.

A rabbit monospecific antibody for mouse beta-spectrin was used to screen a mouse anemic spleen cDNA expression library. A mouse beta-spectrin cDNA clone was isolated and identified by its ability to make mouse beta-spectrin-like antigens in Escherichia coli. This clone was used to probe total RNA from various mouse tissues. Anemic spleen RNA showed two strongly hybridizing RNA species of appro...